Journal: Neural regeneration research

Article Title: Acetyl-11-keto-beta-boswellic acid promotes sciatic nerve repair after injury: molecular mechanism.

doi: 10.4103/1673-5374.339494

Figure Lengend Snippet: Figure 3 ｜ Gene and protein verification in sciatic nerve injury in rats treated with AKBA. (A) mRNA expression levels (relative to GAPDH) of NGFR (A1), NGF (A2), BDNF (A3), and MPO (A4) in the rat sciatic nerve using qPCR. (B) Protein expression levels (relative to GAPDH) of NGFR (B2), NGF (B3), BDNF (B4), and MPO (B5) in the rat sciatic nerve by western blot assay. Data are expressed as the mean ± SD (n = 3 rats/group) and were analyzed using a one-way analysis of variance followed by the least significant difference test. BDNF: Brain derived neurotrophic factor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; MPO: myeloperoxidase; NGF: nerve growth factor; NGFR: nerve growth factor receptor; qPCR: real-time polymerase chain reaction.

Article Snippet: Supplier BDNF 1:500 Rabbit DF6387 AB_2838350 Affinity Biosciences (Beijing, China) NGFR 1:500 Rabbit DF6821 AB_2838781 Affinity Biosciences MPO 1:500 Rabbit A1374 AB_2760599 ABclonal Technology (Wuhan, China) NGF 1:500 Rabbit A14216 AB_2761076 ABclonal Technology GAPDH 1:1000 Rabbit Bs-2188R AB_11065664 Bioss Biotechnology (Beijing, China) IL-1β 1:400 Rabbit WLH3903 AB_2894981 Wanleibio (Shenyang, China) Pro-IL-1β 1:500 Rabbit WL02257 AB_2894987 Wanleibio TNF-α 1:500 Rabbit WL01581 AB_2894992 Wanleibio β-Actin 1:1000 Rabbit bs-0061R AB_10855480 Bioss Biotechnology BDNF: Brain derived neurotrophic factor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IL-1β: interleukin 1 beta; MPO: myeloperoxidase; NGF: nerve growth factor; NGFR: nerve growth factor receptor; Pro-IL-1β: pro interleukin 1 beta; TNF-α: tumor necrosis factor-alpha.

Techniques: Expressing, Western Blot, Derivative Assay, Real-time Polymerase Chain Reaction

Journal: Neural regeneration research

Article Title: Acetyl-11-keto-beta-boswellic acid promotes sciatic nerve repair after injury: molecular mechanism.

doi: 10.4103/1673-5374.339494

Figure Lengend Snippet: Figure 2 ｜ Genomics analysis after sciatic nerve injury treated with AKBA in rats. (A) Differential gene expression volcano map. (A1) AKBA group vs. Model group. (A2) AKBA group vs. Sham group. (A3) Model group vs. Sham group. (B) KEGG analysis of differential gene expression in the sciatic nerve. (B1) AKBA group vs. Model group. (B2) Heat map of KEGG enrichment analysis of differentially expressed mRNA between the AKBA and Model groups. Each row represents an enriched KEGG pathway, and each column represents differential mRNA expression. The red color indicates that the mRNA was detected in the corresponding KEGG pathway, and vice versa, and the gray color indicates that no enrichment was detected. (B3) The most significantly different gene expression was in the phagosome pathway. A redder color indicates more up-regulation of gene expression in the AKBA group than in the Model group, and a greener color indicates more down-regulation in the AKBA group than in the Model group. (B4) Expression of different genes after normalization (FRKM values). (B5) AKBA group vs. Sham group. (B6) Model group vs. Sham group. Data are expressed as the mean ± SD (n = 5 rats/group). BDNF: Brain derived neurotrophic factor; Cybb: cytochrome B-245 beta chain; C3: complement C3; NGF: nerve growth factor; NGFR: nerve growth factor receptor: MPO: myeloperoxidase; RT1-M2: RT1 class Ib: locus M2; Stx7: syntaxin 7; Tcirg1: T cell immune regulator 1; Thbs2: thrombospondin 2.

Article Snippet: Supplier BDNF 1:500 Rabbit DF6387 AB_2838350 Affinity Biosciences (Beijing, China) NGFR 1:500 Rabbit DF6821 AB_2838781 Affinity Biosciences MPO 1:500 Rabbit A1374 AB_2760599 ABclonal Technology (Wuhan, China) NGF 1:500 Rabbit A14216 AB_2761076 ABclonal Technology GAPDH 1:1000 Rabbit Bs-2188R AB_11065664 Bioss Biotechnology (Beijing, China) IL-1β 1:400 Rabbit WLH3903 AB_2894981 Wanleibio (Shenyang, China) Pro-IL-1β 1:500 Rabbit WL02257 AB_2894987 Wanleibio TNF-α 1:500 Rabbit WL01581 AB_2894992 Wanleibio β-Actin 1:1000 Rabbit bs-0061R AB_10855480 Bioss Biotechnology BDNF: Brain derived neurotrophic factor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IL-1β: interleukin 1 beta; MPO: myeloperoxidase; NGF: nerve growth factor; NGFR: nerve growth factor receptor; Pro-IL-1β: pro interleukin 1 beta; TNF-α: tumor necrosis factor-alpha.

Techniques: Gene Expression, Expressing, Derivative Assay

Journal: Frontiers in Physiology

Article Title: Different Levels of Expression of the Clock Protein PER and the Glial Marker REPO in Ensheathing and Astrocyte-Like Glia of the Distal Medulla of Drosophila Optic Lobe

doi: 10.3389/fphys.2018.00361

Figure Lengend Snippet: (A) Horizontal section of the medulla of NP6520 -Gal4/UAS-mCD8-GFP flies immunolabeled with anti-PER antibody. The nuclei of GFP-expressing EnGl (frames 1–3) are PER-positive, which is well visible in higher magnification (panels 1–3). The processes of EnGl (arrowheads in panels 1–3) span the medula neuropil (Mn). R, retina; Lc, Lamina cortex; Ln, lamina neuropil; oCh, outer chiasm; Mc, medulla cortex; iCh, inner chiasm. Scale bars: 20 μm for (A) and 10 μm for panel 1, 2, and 3. (B) The nuclei of GFP-positive EnGl show high level of REPO-specific immunofluorescence (arrow). Arrowhead-the EnGl processes. Scale bar: 10 μm. (C) The distal part of the medulla in horizontal section of NP6520 -Gal4/UAS-mCD8-GFP flies immunolabeled with anti-DmMANF antibody. The processes of EnGl are marked with arrows. R, retina; Lc, lamina cortex; Ln, lamina neuropil; Mc, medulla cortex; Mn, medulla neuropil. Scale bar: 20 μm. (D-D”) Higher magnification of EnGl marked in (C) . The GFP-positive processes of EnGl ( D , arrows) are marked with DmMANF (D',D”) . DmMANF is also visible in the perinuclear space of cell bodies in the medulla cortex (D', arrowheads). Scale bar: 10 μm.

Article Snippet: Cryosections of 20 μm thickness were cut and immunolabeled with primary antibodies as follows: mouse anti-REPO Ab (1:40, Developmental Studies Hybridoma Bank), mouse anti-PDF Ab (1:500, Developmental Studies Hybridoma Bank) rabbit anti-GFP Ab (1:1000, Novus Biologicals), mouse anti-GFP Ab (1:1000, Novus Biologicals), rabbit anti-PER Ab (1:1000, a kind gift of R. Stanewsky), and rabbit anti- Drosophila melanogaster MESENCEPHALIC ASTROCYTE-DERIVED NEUROTROPHIC FACTOR or DmMANF Ab (1:500, GenScript, prepared using PolyExpress Premium Antigen-Specific Affinity Purified pAb and tested for specificity on homogenates of DmMANF -deficient flies).

Techniques: Immunolabeling, Expressing, Immunofluorescence